Abstract
Summary The kinetic complexity of the pea genome is deduced from reassociation experiments. The pea genome contains mainly 2 distinct DNA classes. Besides a slow reassociating fraction, representing exclusively unique DNA or sequences with a very low redundancy, a repetitive DNA class is present, containing 3 to 5% instantaneously reannealing DNA («zero time binding» DNA, consisting of palindromic sequences, not following bimolecular reassociation kinetics) and 40% heterogeneous repetitive DNA, not following ideal second order reassociation kinetics. The mean redundancy of this fraction is 23–37 × 10 2 , while the mean sequence redundancy for the very fast reassociating fraction (except zero time binding DNA) reassociated before C 0 t 10 −1 , is 3.8–6.1 × 10 4 . This degree of redundancy is comparable with the redundancy of animal and plant satellite DNA's; however, the pea does not contain a nuclear satellite DNA.
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