Abstract
An effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened for their capacity for protein synthesis and monosaccharide assimilation. Combined yeast cultivation and pectin hydrolysis were found to be an effective method of producing prebiotics. Separate enzymatic hydrolysis and fermentation of SBP resulted in the release of 3.6 g of POS per 100 g d.w., whereas the yield of POS acquired after the combined process was 17.9% higher, giving 4.2 g of POS per 100 g d.w. Introducing the yeast into the process improved hydrolysis performance due to lower enzyme inhibition by mono- and disaccharides. The prebiotic effect of the POS was assessed by in vitro fermentation using individual cultures of gastrointestinal bacteria. The POS in the SBP hydrolysate effectively promoted the growth of lactobacilli and bifidobacteria. A large increase in adherence to Caco-2 cells in the presence of POS was noted for beneficial Lactobacillus brevis strains, whereas pathogenic bacteria and yeast (C. albicans, C. lusitanie, C. pelliculosa), responsible for infections in breeding animals, showed much weaker adhesion.
Highlights
Farm animals, especially in their first few months of life, are exposed to various environmental stresses, often resulting in nutritional problems caused by imbalances in their intestinal consortia of microorganisms
Various yeast strains belonging to the genera Metschnikowia, Scheffersomyces, Kluyveromyces, and Saccharomyces were seeded on media prepared on the basis of sugar beet pulp (SBP) hydrolysates with (B medium) or without (A medium) nitrogen supplementation
According to Patelski et al [15], the ability to absorb di-ammonium hydrogen phosphate depends on the conditions of yeast breeding, the composition of the medium, and the enzymatic apparatus of the given strain (strains within one species may differ in their ability to assimilate (NH4)2HPO4)
Summary
Especially in their first few months of life, are exposed to various environmental stresses, often resulting in nutritional problems caused by imbalances in their intestinal consortia of microorganisms. The associated stress leads to gastritis, and affects their immune systems and increases the risk of pathogenic infections. During this period, significant changes in digestive tract microbiome have been observed [1,2]. One of the strategies for improving animal nutrition is feed supplementation with pectic oligosaccharides (POS). These non-digestible oligosaccharides are commonly extracted from a variety of agricultural by-products, including olive pomace, apple pomace, sugar beet pulp (SBP), potato pulp, and citrus peel waste [5]. A by-product of the sucrose production process used mainly for feeding farm animals, may be a valuable source of POS [5,9]
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