Abstract
Electron probe microanalysis of biological specimens since its introduction in 1957 has seen relatively few applications. Most have involved the analysis of inclusions, precipitates, normal and pathological mineralization and, to a limited extent, localization of inorganic ions or organic components associated with cellular structures. Resolving powers of 1 to 3 microns, relatively poor X-ray detectors, and lack of appropriate specimen preparation procedures, which affect both the spatial resolution and quantitative analysis, are some of the reasons for the limited application of this method. The need, therefore, for a higher resolution instrument to render biological structural details visible and the measured X-ray intensity quantitative is obvious.
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