Abstract

Phycocyanin, the main pigment substance, is abundant in Nostoc sphaeroides. However, the thermal stability of phycocyanin is insufficient, so the processing and storage of Nostoc sphaeroides remain a critical problem. In this study, we have developed an innovative pasteurization technology: Radio frequency combined hot water pasteurization (RFCHWP) in order to improve the product quality of Nostoc sphaeroides. The results showed that RFCHWP technology exhibited good thermal uniformity and was effective in killing microorganisms in Nostoc sphaeroides. Compared to high-pressure steam sterilization (HPSS), heating by RFCHWP for less than 20 min had little effect on the color and texture of Nostoc sphaeroides samples. The ascorbic acid content of Nostoc sphaeroides decreased to 18.31 and 17.45 mg/100 g in the RFCHWP 30 min and HPSS samples, respectively. The phycocyanin content of Nostoc Sphaeroides in HPSS samples decreased by 32.89% compared with that in the RFCHWP samples. Phycocyanin was stable at a temperature of 50 °C and pH of 6–8. The results of this study showed that RFCHWP technology could be used as a bactericidal method for Nostoc sphaeroides with better product quality.

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