Combined preconditioning with tetra-(1-vinylimidazole) cobalt dichloride

Abstract

The aim of this study was to investigate the vinylimidazole derivative as pharmacological preconditioning in experimental acute hypoxia and cerebral ischemia. All experiments were conducted with mice and rats. Acute hypoxia with hypercapnia was modeled by placing the mice in a glass with a glass lid shtanglazy 250 ml. Acute hypobaric hypoxia was performed putting animals under a glass cope, where from the Kamovsky pump pumped up the air which is equal to the altitude of 5000 meters (moderate hypoxia) and 11 000 meters (severe hypoxia). Cerebral ischemia in rats was modeled by one-stage bilateral common carotid artery occlusion. Separate pharmacological and hypoxic preconditioning and combined preconditioning by alternately use of pharmacological and hypoxic factors were investigated. It was revealed that combined preconditioning (tetra-(1-vinylimidazole) cobalt dichloride 30 mg/kg + moderate hypobaric hypoxia) enhance the resistance of the animal organism to acute hypoxia in the early period (in 1 hr) and delayed period (in 48 hr) more effective than the separate preconditioning by moderate hypoxia or with the help of tetra-(1-vinylimidazole) cobalt dichloride. The combined preconditioning increase the survival of rats in the early periods and in the delayed periods of cerebral ischemia performance, but doesn’t reduce the severity of neurological deficit in the surviving animals registered with the Stroke Index McGraw scale.