Combined passivators regulate the heavy metal accumulation and antioxidant response of Brassica chinensis grown in multi-metal contaminated soils.

Abstract

Passivation of heavy metals is one of the most efficient techniques to remediate soil pollution. However, passivators with single component are usually unsatisfactory in the case of multi-metal contaminated soils. To resolve this problem, a series of combined passivators containing different ratios of Fe-Mn ore, Fe powder, zeolite, bentonite, etc. were designed and used to study their effects on the growth, heavy metal accumulation, and the antioxidant response of Chinese cabbage (Brassica chinensis L.) as well as the soil available forms of heavy metals in a copper refinery's multi-metal (As, Cd, Pb, Cu) contaminated yellow-brown soil and an artificially contaminated (As, Cd, Pb, Cu) calcareous alluvial soil. The results showed that compared with the control, the addition of combined passivators significantly promoted cabbage growth, with the biomass increase up to 1.77 and 3.54 times in yellow-brown soil and calcareous alluvial soil, respectively. The activity of antioxidant enzymes (SOD, CAT, POD) and the content of malondialdehyde (MDA) and glutathione (GSH) decreased, while the chlorophyll content increased significantly, as compared with no passivators. In addition, passivator application decreased As, Cd, Pb, and Cu contents in shoots and roots by 34.8%, 45.6%, 34.9%, and 11.1% and 49.2%, 63.8%, 38.6%, and 46.4% in yellow-brown soil and by 29.8%, 27.3%, 26.8%, and 25.5% and 45.8%, 55.2%, 61.8%, and 5.7% in calcareous alluvial soil, respectively. Besides, the content of soil available heavy metals was reduced by 8.0-17.1% in yellow-brown soil and 3.3-19.1% in calcareous alluvial soil after the application of passivators. The results indicated that the combined passivators formulated in this experiment could efficiently reduce the content of the multi-metals in cabbage and relieve the oxidant stress and could be used as a way to remediate multi-metal polluted soils.