Combined metabolomic and proteomic analyses reveal the aerobic and respirative life-style of Lactobacillus plantarum SXC48

Abstract

O2 is considered one of the main factors affecting the survival of lactic acid bacteria used in the production of a variety of fermented foods and biotechnology industries. The aim of this study was to systematically investigate the differences of Lactobacillus plantarum SXC48 under aerobic, respiratory (aerobic condition with respiratory cofactors-heme and menaquinone supplementation) and anaerobic conditions through quantitative proteomic and metabonomic analyses. Results showed that the growth of SXC48 under respiratory condition was better. O2 impaired the carbohydrate metabolism, especially the citrate cycle pathway. A major metabolic shift was observed after supplementing respiratory cofactors, leading to increased expression of proteins mediating cellular oxidant detoxification and antioxidant activity as well as the production of more nucleotides and less l-lactate. The findings confirmed that respiration reduced the detrimental effect of O2 through the regulation of central carbohydrate, which may be useful for the development of competitive starter or probiotic cultures.