Combined exogenous selenium and biochemical fulvic acid reduce Cd accumulation in rice.

Abstract

Paddy soil Cd contamination and the related accumulation risk in rice grains have attracted global attention. The application of selenium and humic substances is considered to be a cost-effective Cd mitigation measure. However, the effect of a combined application of the two materials remains unclear. Therefore, a 2-season pot experiment was conducted, wherein sodium selenite (Se) and biochemical fulvic acid (BFA) were applied alone and together. Paddy soils with two levels of Cd contamination were used. The results indicate that Se application alone considerably decreased the rice grain Cd content by 36.1-48.7% compared to the control rice grain Cd concentration, which was above the food safety limit (0.2 mg kg-1). Although the application of BFA alone decreased the soil pH, it also increased the soil CaCl2 extractable Cd content by 0.2 to 19.3% and had a limited effect on Cd in the rice grains. The combined application of Se and BFA did not affect the soil pH or the CaCl2 extractable Cd, and more effectively reduced the Cd contents of the rice grains by 50.2 to 57.1%, except for the control rice grain Cd content, which was below the limit. The combined application of Se and BFA also inhibited Se accumulation in rice grains, maintaining the Se content at a safe level (0.33-0.58 mg kg-1) compared to Se application alone. The effects of reducing the Cd content of rice grains while safely increasing their Se contents could persist for at least two seasons. Therefore, the combined application of Se and BFA should be recommended to mitigate Cd contamination risks in Cd-contaminated paddy soil.