Combined Effects of Resveratrol and Vitamin E From Peanut Seeds and Sprouts on Colorectal Cancer Cells.

Chunfeng Wang,Fangyu Wang,Qiuying Yu,Na Wang,Na Li

doi:10.3389/fphar.2021.760919

Abstract

Resveratrol (RES) and Vitamin E (VE) are anti-cancer active ingredients with relatively high content in peanut seeds and sprouts. This study aimed to determine the synergistic inhibitory effect of RES and VE on colorectal cancer. Using 5-FU as a positive drug control, the effect of RES combined with VE on HCT-8 cells was determined, and cell viability was detected using the cell-counting kit 8 (CCK8) method. Cell morphology changes were observed using optical microscopy. Cell migration ability was evaluated by the scratch test, while cell colonies were determined by the cloning test formation ability. Apoptosis status was assessed by flow cytometry and nuclear staining by DAPI, and the expression level of apoptosis-related proteins was determined by western blotting. Compared with the single component group, the RES combined with VE group significantly inhibited the growth and proliferation of HCT-8 intestinal cancer cells in vitro. The RES combined with VE group had a greater impact on cell morphology changes and cell colony formation and significantly reduced cell migration ability and intestinal cancer cell apoptosis (p < 0.05). Additionally, combined treatment with RES and VE significantly upregulated the expression of pro-apoptotic proteins BAX, caspase-3, caspase-8, and caspase-9, and downregulated the expression of anti-apoptotic protein BCL-2, compared to the single component treatment. RES combined with VE is effective in promoting intestinal cancer cell apoptosis. This study demonstrated the significant positive synergy of RES and VE on HCT-8 cells, providing a new perspective for more effective use of RES.

Highlights

Colorectal cancer is one of the most common cancers, and it is the third most common cancer in men and the second in women
The present study determined the effect of different RES and Vitamin E (VE) concentrations on HCT-8 and investigated the effect of RES combined with VE on cell proliferation
After 24 and 48 h of treatment, the combined effect significantly reduced the IC50 values of cell viability compared with single doses (p < 0.05) (Table 1), indicating that RES + VE inhibited the proliferation of cancer cells better than the single component group

Summary

Introduction

Colorectal cancer is one of the most common cancers, and it is the third most common cancer in men and the second in women (van Harten-Gerritsen et al, 2015; Farinetti et al, 2017). In 2018, among all cancers globally, colorectal cancer ranked third in incidence (more than 1.8 million new cases) and second in mortality (more than 860,000 deaths) (Bray et al, 2018). The current standard treatments for colon cancer include surgery, chemotherapy, targeted therapy, and radiotherapy (Zullig et al, 2016). It is necessary to focus on the molecular mechanism of colon cancer tumorigenesis to determine specific targets for drug development, to help develop natural and effective anti-cancer drugs (Auclin et al, 2017), and provide a new perspective for solving human anti-cancer problems.

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