The action mechanism of resveratrol against human colorectal adenocacinoma

Abstract

Objective To illustrate the anticancer property induced by resveratrol (RSV), extracted from Chinese Medicine Polygonum cuspidatum, and provide theoretical basis for the clinical use. Methods Human colorectal adenocarcinoma cell HCT-15 was treated with different concentrations of resveratrol, and cells were then observed under the light microscope to analyze the morphological change. Cell counting kit-8 (CCK-8) assay was used to detect the inhibition ratio of HCT-15 cell proliferation. Immunoblotting was used to detect the changes of apopatosis related markers. Null mice injected with HCT-15 cells were used to detect the pharmacological activity of RSV in vivo. Results After RSV treatment, cell number was reduced with the increased dose of RSV, cell space was increased, and shrinkage was seen. After treatment with 0, 50, 100 μmol/L RSV for 24 h, the survival rate of HCT-15 cells was 100%, 68% and 39% respectively. RSV treatments for 24 h inhibited proliferation and induced HCT-15 cells to death by an 50% inhibitory dose (IC50) at 72 μmol/L. Immunoblotting analysis showed that RSV induced apoptosis of HCT-15 cells mainly through mitochondrial pathway. Null mice xenogfaft study showed that RSV treatment could decrease the volume of tumor in vivo, and tumor inhibition rate by RSV at 0, 50, 100 μmol/L of RSV was 0.0%, 43.6%, and 68.6% respectively. Conclusion RSV inhibits the human colorectal adenocarcinoma cells in vitro proliferation significantly mainly probably by inhibiting apoptosis. RSV can effectively inhibit tumor growth in vivo. Key words: Polygonum cuspidatum; Resveratrol; Colorectal adenocarcinoma; Pharmacological activity.