Abstract
The use of circulating microRNAs as biomarkers opens up new opportunities for the diagnosis of cardiovascular diseases because of their specific expression profiles. The aim of the present study was to identify circulating microRNAs in human plasma as potential biomarkers of heart failure and related diseases. We used real-time quantitative PCR to screen microRNA in plasma samples from 62 normal controls and 62 heart failure samples. We found that circulating miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 expressed differently between healthy controls and heart failure patients. Plasma levels of miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 were unaffected by hemolysis. Correlation analysis showed any two of these miRNAs possess a strong correlation, indicating a possibility of combined analysis. MiR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 could be combined in two or three or more combinations. The results suggest that miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 may be a new diagnostic biomarker for heart failure and related diseases.
Highlights
In recent years, cardiovascular diseases still rank the first killer of human death and heart failure accounts one of them
We found that plasma levels of miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 were not affected by hemolysis, age, and gender when used to diagnose heart failure. miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 may be a new biomarker for the diagnosis of heart failure and related diseases
These microRNAs have potential as diagnostic and prognostic biomarkers and will be used in clinical diagnosis and treatment of heart failure diseases in conjunction with existing gold standards
Summary
Cardiovascular diseases still rank the first killer of human death and heart failure accounts one of them. The trend of younger heart failure group rises substantial in recent years, no enough research attention has been paid to the younger generation [1–5]. MicroRNAs (miRNAs) are a class of non-protein encoded small RNAs that widely exist in eukaryotes and have a length of 21–25 nucleotides. They are highly stable in the blood circulation and can regulate gene expression in a sequence-specific manner. They play an important role in development, apoptosis, metabolism and human diseases. The physiological and pathological regulation mechanism of miRNA is a new discipline which has been highly valued in recent years [10–12]
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