Abstract

BackgroundPhotobiomodulation with low-intensity laser (LIL) and chondroitinase ABC (ChABC) can repair damaged muscle tissue, so the aim of this study was to investigate the effect of co-administration of these two factors on anal sphincter repair in rabbits.MethodsMale rabbits were studied in 5 groups (n = 7): Control (intact), sphincterotomy, laser, ChABC and laser + ChABC. 90 days after intervention were evaluated resting and maximum squeeze pressures, number of motor units, collagen amount, markers of muscle regeneration and angiogenesis.ResultsResting pressure in the Laser + ChABC group was higher than the sphincterotomy, laser and ChABC groups (p < 0.0001). Maximum squeeze pressure in the all study groups was higher than sphincterotomy group (p < 0.0001). In the laser + ChABC and ChABC groups, motor unit numbers were more than the sphincterotomy group (p < 0.0001). Collagen content was significantly decreased in the laser (p < 0.0001) and laser + ChABC groups. ACTA1 (p = 0.001) and MHC (p < 0.0001) gene expression in the Laser + ChABC group were more than the laser or ChABC alone. VEGFA (p = 0.009) and Ki67 mRNA expression (p = 0.01) in the Laser + ChABC group were more than the laser group, But vimentin mRNA expression (p < 0.0001) was less than the laser group.ConclusionCo-administration of ChABCs and photobiomodulation with LIL appears to improve the tissue structure and function of the anal sphincter in rabbits more than when used alone.

Highlights

  • Photobiomodulation with low-intensity laser (LIL) and chondroitinase ABC (ChABC) can repair dam‐ aged muscle tissue, so the aim of this study was to investigate the effect of co-administration of these two factors on anal sphincter repair in rabbits

  • Photobiomodulation with LIL (p < 0.0001) and administration of Chondroitin ABC Lyase (ChABC) (p < 0.0001) led to a significant improvement in resting pressure compared to the sphincterotomy group

  • Co-administration of ChABC + Laser is associated with higher maximum squeeze pressure than laser (p = 0.005) and ChABC (p < 0.0001) alone (Fig. 1)

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Summary

Introduction

Photobiomodulation with low-intensity laser (LIL) and chondroitinase ABC (ChABC) can repair dam‐ aged muscle tissue, so the aim of this study was to investigate the effect of co-administration of these two factors on anal sphincter repair in rabbits. The main treatment for FI is various surgeries such as injection of bulking agents [6], Sarveazad et al BMC Gastroenterology (2021) 21:473 tibial nerve stimulation [7], neuromodulation [8], sacral nerve stimulation [9], and cecostomy [9]. These treatments do not have long-lasting and effective results. LIL can be waking up these satellite cells and entering the proliferative phase [17,18,19]

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