Abstract
Genetic information stored in DNA sequences is translated into protein by linking a triplet nucleotide sequence and an amino acid. Because the frames of the triplets can be configured in three ways, a total of six polypeptides, each with a different sequence, can be produced from a single double-stranded DNA molecule. We recently developed the MolCraft system [reviewed in K. Shiba, J. Mol. Catal. B 18 (2004) xxx], which enables us to make combinatorial polymers of three peptides translated from one strand of a double-stranded DNA molecule. To explore all the information that a single double-stranded DNA molecule encodes, we have now developed a new system, La-MolCraft, in which all six reading frames encoded by both strands are combinatorially polymerized using loop-mediated isothermal amplification of DNA (LAMP) [Nucl. Acids Res. 28 (2000) E63].
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