Abstract
The present work was to investigate the effects of oral supplementation of chondroprotective influence of niacinamide (NA) and undenatured type II collagen (UC-II®) on the inflammation and the joint pain behavior of rats in a monoiodoacetate (MIA)-induced experimental osteoarthritis (OA) model. Forty-nine Wistar rats were allocated into seven groups, namely; 1) control (no MIA); 2) MIA, positive control as 1 mg MIA-induced knee osteoarthritis; 3) MIA+UCII, MIA + UCII at 0.66 mg/kg/BW; 4) MIA+NA40, MIA + NA at 40 mg/kg BW; 5) MIA+NA200, MIA (1 mg) + NA at 200 mg/kg BW; 6) MIA+UCII+NA40 : MIA (1 mg) + UCII at 0.66 mg +NA at 40 mg/kg BW and 7) MIA+UCII+NA200: MIA (1 mg) + UCII at 0.66 mg + NA at 200mg/kg BW. OA outcomes such as gait test (paw area, paw width, stride length) of the knee joint was analyzed. Serum interleukin 1beta (IL-1β), interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor-alpha (TNF-α), cartilage oligomeric matrix protein (COMP), C-reactive protein (CRP), serum malondialdehyde (MDA) and knee joint matrix metalloproteinase-3 (MMP-3), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κβ), and transforming growth factor-beta (TGF-β)-1 levels were analyzed. Analysis of variance (ANOVA) test and post-hoc Tukey test was used for multiple comparisons of the groups. Serum IL-1β, IL-6, TNF-α, COMP, and CRP decreased in rats supplemented with UCII and in combinations with NA compared with control rats (p < 0.05). Rats supplemented with UCII and in combinations with NA reduced serum MDA and knee joint MMP-3, NF-κβ, and TGF-β protein levels (p < 0.05). The UCII supplementation to OA rats decreased the Kellgren-Lawrence and the Mankin scores (p < 0.05) indicating a reduced in OA disease severity. These results suggest undenatured type II collagen combined with niacinamide is effective in reducing OA outcomes.
Published Version
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