Combination of Niacinamide and Undenatured Collagen Type II Modulates Inflammatory Response in Monosodium Iodoacetate‐Induced Osteoarthritis in Rats

Abstract

The present work was to investigate the effects of oral supplementation of chondroprotective influence of niacinamide (NA) and undenatured type II collagen (UC-II®) on the inflammation and the joint pain behavior of rats in a monoiodoacetate (MIA)-induced experimental osteoarthritis (OA) model. Forty-nine Wistar rats were allocated into seven groups, namely; 1) control (no MIA); 2) MIA, positive control as 1 mg MIA-induced knee osteoarthritis; 3) MIA+UCII, MIA + UCII at 0.66 mg/kg/BW; 4) MIA+NA40, MIA + NA at 40 mg/kg BW; 5) MIA+NA200, MIA (1 mg) + NA at 200 mg/kg BW; 6) MIA+UCII+NA40 : MIA (1 mg) + UCII at 0.66 mg +NA at 40 mg/kg BW and 7) MIA+UCII+NA200: MIA (1 mg) + UCII at 0.66 mg + NA at 200mg/kg BW. OA outcomes such as gait test (paw area, paw width, stride length) of the knee joint was analyzed. Serum interleukin 1beta (IL-1β), interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor-alpha (TNF-α), cartilage oligomeric matrix protein (COMP), C-reactive protein (CRP), serum malondialdehyde (MDA) and knee joint matrix metalloproteinase-3 (MMP-3), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κβ), and transforming growth factor-beta (TGF-β)-1 levels were analyzed. Analysis of variance (ANOVA) test and post-hoc Tukey test was used for multiple comparisons of the groups. Serum IL-1β, IL-6, TNF-α, COMP, and CRP decreased in rats supplemented with UCII and in combinations with NA compared with control rats (p < 0.05). Rats supplemented with UCII and in combinations with NA reduced serum MDA and knee joint MMP-3, NF-κβ, and TGF-β protein levels (p < 0.05). The UCII supplementation to OA rats decreased the Kellgren-Lawrence and the Mankin scores (p < 0.05) indicating a reduced in OA disease severity. These results suggest undenatured type II collagen combined with niacinamide is effective in reducing OA outcomes.