Abstract
Combination of Autosomal and Y-STR Analysis, an Alternative to Differential Extraction: A Case Study
Highlights
For the last 3-4 yrs, the pendency of the sexual assault cases has become a serious concern to the Court of Law, all over the globe
As the male-DNA contribution is much lower than the female epithelial cell population in gynecological sample, analysis and interpretation of the individual contributions, has always been a challenge for the forensic scientists
As the mixed autosomal profile included both the suspect and the victim, using the qualitative and semi-quantitative binary model of interpretation, the Y-short tandem repeat (STR) analysis confirmed the offender. This method is applicable when a preliminary suspect list is available to the investigative agency to compare with. Since this method is simple and is not influenced either by the azoospermic and vasectomized male samples or mixed samples other than semen viz. male DNA under fingernails of the victim, male touch DNA on the skin of the victim, and the clothing or belongings of a female victim, we propose this combination method may be highly effective in resolving a broad spectrum of cases in the Forensic laboratory
Summary
For the last 3-4 yrs, the pendency of the sexual assault cases has become a serious concern to the Court of Law, all over the globe. We propose our method to be extremely useful to individualize the perpetrator in broad spectrum of forensic cases including the sexual assault, where the male DNA is extremely small, but its contribution is one in five of female part or more in the mixture.
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