Combatting the antigenicity of common ragweed pollen and its primary allergen Amb a 1 with cold atmospheric pressure air plasma

Abstract

Airborne allergens, especially those originating from various types of pollen, significantly compromise the health and well-being of individuals on a global scale. Here, cold atmospheric pressure plasma (CAP) created in ambient air was used to treat highly allergenic and invasive Ambrosia artemisiifolia pollen. Immunoassays were used to evaluate the impact of CAP on the principal A. artemisiifolia allergen Amb a 1, demonstrating that > 90 % reduction in antigenicity could be achieved. Chemical analyses using Fourier Transform infrared revealed that CAP induced significant alterations to proteins on the surface of pollen grains, resulting in a 43 % increase in the amide I peak area and a 57 % increase in the amide II peak area. These findings were corroborated by Raman and X-ray photoelectron spectroscopy, which indicated that the protein modifications induced by CAP were due to carbonylation and nitration/nitrosylation processes. Beyond protein transformations, CAP also induced notable oxidation and modification of lipid-like compounds, polysaccharides and sporopollenin. Evident transformations at the chemical level translated into morphological changes at the grain surface, manifesting as increased roughness via significant outer-layer etching. These findings underscore the potential of CAP technology as a viable approach for mitigating against the allergenicity of pollen, providing a deeper understanding into the underlying chemical mechanisms.