Colorimetric aptasensor for ochratoxin A detection based on enzyme-induced gold nanoparticle aggregation

Abstract

An innovative colorimetric method based on enzyme-induced gold nanoparticle aggregation was developed to detect the activity of alkaline phosphatase (ALP), and it was further applied to construct an aptasensor to monitor ochratoxin A (OTA) concentrations. In the presence of ALP, the substrate ascorbic acid 2-phosphate was hydrolyzed to generate ascorbic acid (AA). Subsequently, reduction of MnO2 nanosheets by AA produced manganese ions, which mediated gold nanoparticle aggregation. The color of the detection solution changed from brown–red to purple to blue as the ALP concentration increased, and a detection limit of 0.05 U·L−1 was achieved. Furthermore, this strategy was successfully utilized to devise a target-responsive aptasensor for colorimetric detection of an important mycotoxin, OTA, which causes food poisoning and has various toxic effects on humans. The proposed method offers high sensitivity with a detection limit as low as 5.0 nM together with high specificity. When applied to analyze red wine and grape juice samples, no complex sample pretreatment or bulky instruments were required. Overall, a colorimetric platform based on enzyme-induced gold nanoparticle aggregation was successfully established to improve the simplicity and sensitivity of ALP and OTA detection. This platform appears highly promising for mycotoxin-related food safety monitoring.