Colonization of Germ-Free Piglets with Mucinolytic and Non-Mucinolytic Bifidobacterium boum Strains Isolated from the Intestine of Wild Boar and Their Interference with Salmonella Typhimurium.

Alla Splichalova,Igor Splichal,Radko Pechar,Zdislava Splichalova,Vera Neuzil Bunesova,Jiri Killer,Hana Subrtova Salmonova,Eva Vlkova

doi:10.3390/microorganisms8122002

Abstract

Non-typhoidal Salmonella serovars are worldwide spread foodborne pathogens that cause diarrhea in humans and animals. Colonization of gnotobiotic piglet intestine with porcine indigenous mucinolytic Bifidobacterium boum RP36 strain and non-mucinolytic strain RP37 and their interference with Salmonella Typhimurium infection were compared. Bacterial interferences and impact on the host were evaluated by clinical signs of salmonellosis, bacterial translocation, goblet cell count, mRNA expression of mucin 2, villin, claudin-1, claudin-2, and occludin in the ileum and colon, and plasmatic levels of inflammatory cytokines IL-8, TNF-α, and IL-10. Both bifidobacterial strains colonized the intestine comparably. Neither RP36 nor RP37 B. boum strains effectively suppressed signs of salmonellosis. Both B. boum strains suppressed the growth of S. Typhimurium in the ileum and colon. The mucinolytic RP36 strain increased the translocation of S. Typhimurium into the blood, liver, and spleen.

Highlights

The coevolution of the host and its microbiota developed a mutually-beneficial relationship between them, and the microbial community became indispensable for the host’s metabolism and health [1]
Our work aimed to study a monocolonization of the intestine of newborn germ-free piglets with mucinolytic and non-mucinolytic strains of Bifidobacterium boum, its impact on the modification of the intestinal barrier, interference of bifidobacteria with S
We found that bifidobacterial counts of both strains of B. boum in monocolonized piglets or in the piglets subsequently infected with S

Summary

Introduction

The coevolution of the host and its microbiota developed a mutually-beneficial relationship between them, and the microbial community became indispensable for the host’s metabolism and health [1]. Bifidobacteria are the most abundant enteric bacteria in the vaginally born infant intestine [14,15]. They confer their beneficial effect by the saccharolytic activity toward glycans abundant in the infant intestine [16]. They suppress pathogenic microorganisms [14,17,18,19]. According to recently corrected counts, the total number of bacterial cells is comparable with the number of the host body cells [26]

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Conclusion