Colonization activity of conditionally pathogenic microorganisms and features of biofilm formation on the surface of thermoplastic polymer material

Abstract

Was to assess the microbial colonization and biofilm-forming activity of conditionally pathogenic microorganisms in vitro to samples of acrylic-free thermoplastic polymer material and to assess the possibility of its use for the manufacture of removable tooth splinting structures by injection method. The reference strains S. aureus, S. epidermidis, S. pyogenes, E. faecalis, E. coli, and C. albicans. Biofilm-forming activity was assessed by the level of ethanol extraction of a 0.1% aqueous solution of gentian violet by measuring on a PowerWave X microplate reader (USA). A low degree of severity of microbial colonization and sufficient colonization resistance to the formation of a microbial biofilm of E. coli (0.374±0.056 cu opt. Pl.), S. aureus (0.272±0.039 cu. Opt. Pl.), S. epidermdis (0.299±0.028 cu opt. Pl.), S. ryogenes (0.399±0.069 cu opt. Pl.), E. faecalis (0.401±0.089 cu opt. Pl.). Moreover, C. albicans strains form a more pronounced biofilm (0.425±0.104 cu opt. Pl.) in comparison with other strains studied in the experiment. Samples from a thermoplastic polymer based on polyoxymethylene have satisfactory biomedical characteristics and can be used at the stages of dental orthopedic treatment of patients without oral candidiasis.