Abstract
The use of Vibrio strains as probiotics in Indonesia is very rarely reported. The purpose of this study is to select and develop Vibrio probiotic strains that can increase the rate of abalone growth. The ability of bacterial isolates to colonize the abalone digestive tract is one of the important parameters in the selection of probiotic candidates. The research method consisted of 3 main stages namely modification of Vibrio sp isolates to rifampicin mutants, manufacture of gnotobiotic abalone and colonization capacity test. The treatment given in the colonization capacity test is a type of bacteria that consists of Vibrio sp. line Abn1.2RfR, Alg3.1RfR, combination of Abn1.2RfR + Alg3.1RfR, and control without the addition of Vibrio sp. Each type of bacterial treatment was entered in a 10 liter aquarium capacity containing 10 abalone size ± 3.5 cm. Feed in the form of Gracilaria cake containing Vibrio sp. (final concentration of 107 cfu / mL) was given at the beginning of the study. Abalone is observed for 24, 48 and 72 hours without water replacement. Then count the total number of bacteria and the number of agarolytic bacteria. The results showed that the growth characteristics of wild-type Vibrio with mutant Vibrio were not different.Thus it is expected that the use of the two mutant isolates for in vivo testing has the same effectiveness as the wild type.The capacity of colonization of a single isolate or a combination of rifampicin mutant Vibrio continues to decrease with increasing time of observation. Nevertheless, the percentage of mixed isolates remained higher than that of single isolates during the observation time
Highlights
IntroductionInhibitor compounds produced by certain Vibrio isolates can reduce other community members such as Alfa-proteobacteria and Alteromonas (Lin et al, 2018)
Among marine bacteria, Vibrio is an important producer of antibiotics
2020, Volume 6, Issue 2, 173-177 this bacterial group has a number of characters needed by a probiotic (Ortiz-Carillo et al, 2015) in an aquaculture system, including being able to compete with other bacteria (Thompson et al, 2010), add nutrition by providing essential nutrients, can improve digestibility by releasing essential enzymes (Lin et al, 2018) has the ability to colonize the digestive tract and host body (Gomez-Gil et al, 2014), and can produce substances that inhibit the growth of opportunistic pathogenic bacteria (Pereire et al, 2018)
Summary
Inhibitor compounds produced by certain Vibrio isolates can reduce other community members such as Alfa-proteobacteria and Alteromonas (Lin et al, 2018). This genus plays an important role in the nutritional cycle in aquatic environments through the breakdown of organic matter. A strain of bacteria can be functional as a probiotic if it has a high capacity to be able to live and colonize the digestive tract of organisms under various conditions of the aquatic environment. In this study a capacity test for the colonization of Vibrio spp Abn1.2 and Alg3.1 strains in the digestive tract of the gnotobiotic abalone was carried out
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