Abstract
Abstract Previously, we used a monoclonal antibody, designated H5, to isolate a tumor-associated antigen (TAA) expressed by 9L, a gliosarcoma line isolated from Fischer 344 (F344) rats. This TAA was tentatively identified as vimentin via mass spectrometry. In this project, we identified the location of this TAA and vimentin on 9L gliosarcoma and normal rat glial cells using confocal fluorescent microscopy. Vimentin and the TAA always co-localized which suggests that the TAA bound by H5 is a moiety on the vimentin protein. In addition, 9L expressed an increased level of vimentin compared to normal rat glial cells. Glial cells were examined from neonatal, 6 week, and adult rats. The glial cells expressed less vimentin than 9L gliosarcoma tumor cells at all ages. These results suggest that vimentin is upregulated as part of the tumor phenotype giving a possible mechanism for detecting transformed cells of this tumor type.
Published Version
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