Abstract
ABSTRACTThe histochemical property of colloid was studied in two cases of colloid milium. Alcian blue, azure A, and periodic acid Schiff were used to stain mucopolysaccharides. Bromphenol blue was employed for detection of basic proteins. In a further attempt to identify various polyanions histochemically, staining was carried out with alcian blue containing various concentrations of electrolytes. Methylation, saponification, mild acid hydrolysis, and digestion with streptomyces or testicular hyaluronidase, or chondroitinase ABC were also employed. The results obtained indicate that the colloid in the present cases contains sulfated acid mucopolysaccharide, probably chondroitin sulfate C.
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