Abstract
The histochemical property of the hyaline material was studied in a case of hyalinosis cutis et mucosae. Alcian blue, azure A and periodic acid Schiff were used for staining mucopolysaceharides. Bromphenol blue was employed for detection of basic proteins. In a further attempt to identify various polyanions histochemically, staining was carried out with alcian blue containing different concentrations of electrolytes. Methylation, saponification, mild acid hydrolysis and digestions by hyaluronidase and sialidase were also employed. The results obtained suggest that the hyaline material found in the case of hyalinosis cutis et mucosae contains a sialic acid-containing mucoprotein.
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