Abstract
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Highlights
A description is given of a technique of microvolume blood sample collection into heparinized glass capillaries from which the samples are immediately transferred to diluting solution to obtain a basic 100-fold dilution
The results show that air-drying of the serum samples and their storage for 7 days at 20°C had an adverse effect on the sensitivity of the detection of antiviral antibodies (Table 1)
The technique of blood sample collection for Enzyme-Linked Immunosorbent Assay (ELISA) and RIA into capillaries described in the present study has been previously verified by examination of several thousands of samples
Summary
Jurak: Collection of Microvolume Blood Samples into Glass Capillaries for the Detection of Antibody Against Aujeszky's Disease Virus in Pigs by Enzyme-Linked Immunosorbent Assay (ELISA) and Solid-Phase Radioimmunoassay (RIA). A description is given of a technique of microvolume blood sample collection into heparinized glass capillaries from which the samples are immediately transferred to diluting solution to obtain a basic 100-fold dilution. The samples are ready for serological examination by ELISA and RIA without further processing at the laboratory, a distinct advantage over present methods of blood collection for serological examination. The technique was tested and verified in pigs by comparing the sensitivity of the detection of antibody against Aujeszky's disease virus by ELISA, RIA and the virus neutralization (VN) test. The antibody titres by ELISA using HRP-RIgaSwIgG and HRP-protein A weflt 51.9-fold and 10-fold higher, respectively, than those obtained by the VN test. Further application of the technique for the detection of antibody by ELISA and RIA in veterinary and human medicine is discussed
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