Abstract
Five different proteases were used to hydrolyze the swim bladders of Nibea japonica and the hydrolysate treated by neutrase (collagen peptide named SNNHs) showed the highest DPPH radical scavenging activity. The extraction process of SNNHs was optimized by response surface methodology, and the optimal conditions were as follows: a temperature of 47.2 °C, a pH of 7.3 and an enzyme concentration of 1100 U/g, which resulted in the maximum DPPH clearance rate of 95.44%. Peptides with a Mw of less than 1 kDa (SNNH-1) were obtained by ultrafiltration, and exhibited good scavenging activity for hydroxyl radicals, ABTS radicals and superoxide anion radicals. Furthermore, SNNH-1 significantly promoted the proliferation of HUVECs, and the protective effect of SNNH-1 against oxidative damage of H2O2-induced HUVECs was investigated. The results indicated that all groups receiving SNNH-1 pretreatment showed an increase in GSH-Px, SOD, and CAT activities compared with the model group. In addition, SNNH-1 pretreatment reduced the levels of ROS and MDA in HUVECs with H2O2-induced oxidative damage. These results indicate that collagen peptides from swim bladders of Nibea japonica can significantly reduce the oxidative stress damage caused by H2O2 in HUVECs and provides a basis for the application of collagen peptides in the food industry, pharmaceuticals, and cosmetics.
Highlights
Redox reactions are basic physiological and chemical reactions that are constantly carried out in the human body
The defatted swim bladders were used to hydrolyze the protein for 4 h at the optimal temperature and pH of each enzyme
The hydrolysate was boiled for 10 min
Summary
Redox reactions are basic physiological and chemical reactions that are constantly carried out in the human body. During metabolism in the human body, large amounts of reactive oxygen species (ROS) are generated and the antioxidant defense system in the body removes active oxygen free radicals in order to prevent damage to the body [1,2]. A healthy human body has an efficient and dynamic antioxidant defense system where the generation and removal of oxidative radicals in the human body are in a dynamic balance. Once the human body generates excessive reactive oxygen radicals or the antioxidant defense system becomes inefficient, this dynamic balance is disturbed, which generates a state of oxidative stress, leading to aging and a number of chronic diseases, such as diabetes and coronary arteriosclerosis [1,3].
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