Colicin E2 Expression inLactobacillus brevisDT24, A Vaginal Probiotic Isolate, against UropathogenicEscherichia coli

Disha Trivedi,Sriram Seshadri,Prasant Kumar Jena

doi:10.1155/2014/869610

Abstract

Novel therapeutic approaches are needed to combat the urinary tract infection in women. During menstruation elevated protein concentration and increase in oxygen and carbon dioxide concentrations with decrease in vaginal Lactobacilli all together contribute to urinary tract infections. Lactobacillus species are a predominant member of the vaginal microflora and are critical in the prevention of a number of urogenital diseases. In order to increase antimicrobial potential of vaginal Lactobacilli, bacteriocin colicin E2 which has specific activity against uropathogenic Escherichia coli has been overexpressed in vaginal probiotic Lactobacillus brevis DT24. Recombinant Lactobacillus brevis DT24 expressing colicin E2 showed much higher inhibitory activity against uropathogenic Escherichia coli than wild type L. brevis DT24 in vitro. Efficacy of probiotic Lactobacillus brevis DT24 expressing colicin E2 protein is required for further in vivo evaluation.

Highlights

Urinary tract infection (UTI) is the most widespread infection in women worldwide after intestinal infection [1]
UTI can be dangerous in pregnant women in whom it has been shown that up to 50% of those with asymptomatic bacteriuria (ABU) leads to develop pyelonephritis
Escherichia coli DH5α, Escherichia coli BL21 D3, and Escherichia coli NCTC 50133 were routinely cultured in Luria Bertani (LB) broth and agar at 37∘C for 16 to 24 h and uropathogenic Escherichia coli MTCC 729 (Microbial Type Culture Collection, Chandigarh) was routinely cultured in nutrient broth and agar at 37∘C for 16 to 24 h

Summary

Introduction

Urinary tract infection (UTI) is the most widespread infection in women worldwide after intestinal infection [1]. Recurrent UTI (rUTI) is a common syndrome in young healthy women. Previous studies suggest that 27% to 44% of women, who experienced an initial UTI, develop rUTI [5, 6]. UTI can be dangerous in pregnant women in whom it has been shown that up to 50% of those with asymptomatic bacteriuria (ABU) leads to develop pyelonephritis. These women experience higher rates of intrauterine growth restriction and low birth weight infants. The presence of a UTI has been shown to increase the risk of preterm labor, preterm birth, pregnancy-induced hypertension, preeclampsia, amnionitis, and anemia [8]

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