Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity

Mahendran Vanaja,Gurusamy Annadurai

doi:10.1007/s13204-012-0121-9

Abstract

The utilization of various plant resources for the biosynthesis of metallic nanoparticles is called green nanotechnology, and it does not utilize any harmful chemical protocols. The present study reports the plant-mediated synthesis of silver nanoparticles using the plant leaf extract of Coleus aromaticus, which acts as a reducing and capping agent. The silver nanoparticles were characterized by ultraviolet visible spectroscopy, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and the size of the silver nanoparticles is 44 nm. The bactericidal activity of the silver nanoparticles was carried out by disc diffusion method that showed high toxicity against Bacillus subtilis and Klebsiella planticola. Biosynthesis of silver nanoparticles by using plant resources is an eco-friendly, reliable process and suitable for large-scale production. Moreover, it is easy to handle and a rapid process when compared to chemical, physical, and microbe-mediated synthesis process.

Highlights

Nanotechnology is likely to prominently manipulate science, economy and day-to-day life in this twenty-firstThe physical (Xu et al 2008) and chemical processes (Wang et al 2005) are the classical general methods used for the fabrication of nanoparticles, but these methods are not environmentally benign (Dubey et al 2010) and due to the presence of some toxic metals in the synthesis process that may create some dicey effects in biomedical applications (Bar et al 2009a)
The present study reports the plantmediated synthesis of silver nanoparticles using the plant leaf extract of Coleus aromaticus, which acts as a reducing and capping agent
The leaves of C. aromaticus was used for biosynthesis of silver nanoparticles, biosynthesized silver nanoparticles are characterized by UV–vis spectroscopy, X-ray diffraction (XRD), EDAX, and the capping agent for the silver nanoparticles synthesis was confirmed by Fourier transform infrared spectroscopy (FTIR)

Summary

Introduction

Nanotechnology is likely to prominently manipulate science, economy and day-to-day life in this twenty-firstThe physical (Xu et al 2008) and chemical processes (Wang et al 2005) are the classical general methods used for the fabrication of nanoparticles, but these methods are not environmentally benign (Dubey et al 2010) and due to the presence of some toxic metals in the synthesis process that may create some dicey effects in biomedical applications (Bar et al 2009a). Known as Indian Borage belongs to the Lamiaceae family, and it is a commonly available medicinal plant, which is used for the biofabrication of silver nanoparticles It is a tender, fleshy, highly aromatic pubescent herb with distinctive smelling medicinal plant contains many phytochemicals such as carvacrol (monoterpenoid), caryophyllene (bicyclic sesquiterpene) and patchoulene and flavonoids like quercetin, apigenin, luteolin, salvigenin, and genkwanin (Ram and Mehrotra 1970). Fleshy, highly aromatic pubescent herb with distinctive smelling medicinal plant contains many phytochemicals such as carvacrol (monoterpenoid), caryophyllene (bicyclic sesquiterpene) and patchoulene and flavonoids like quercetin, apigenin, luteolin, salvigenin, and genkwanin (Ram and Mehrotra 1970) It is used for the treatment of malarial fever, hepatopathy, renal and vesical calculi, cough, chronic asthma, hiccough, bronchitis, anthelmintic, colic and convulsions (Nadkarni 1996). The bactericidal effect of silver nanoparticles was analyzed by disc diffusion method

Methods

Results

Conclusion