Cold Treatment Modulates Changes in Primary Metabolites and Flowering of Cut Flower Tulip Hybrids

Abstract

Tulip is one of the most important bulbous genera in the world’s floriculture. It is known that cold exposure of bulbs before planting is required to break the bulb dormancy and to promote the plant’s flowering. Preparation procedures performed by breeders differ in the duration and the thermal level, and the choice of the procedure depends on the genotype’s sensitivity to temperature; however, little is known about the metabolic responses underlying the different behaviours of the numerous commercial hybrids. We evaluated the influence of two bulb-preparation procedures, 15–18 weeks at 5 ÷ 9 °C, and 9–14 weeks at 2 ÷ 5 °C, in two hybrids of tulip (Tulipa gesneriana L.), ‘Royal Virgin’ and ‘Ad Rem’, grown hydroponically in a floating system. Tulip plants of the two hybrids responded differently to bulb exposure to low temperatures in terms of early flowering, as this was unaffected by the preparation procedure in ‘Royal Virgin’ (27.1 days from transplanting, on average), while it was earlier after treatment at higher temperatures compared with lower temperatures in ‘Ad Rem’ (24.1 vs. 26.7 days at 5 °C vs. at 9 °C). This different flowering earliness may be related to the diverse metabolic responses enacted by the bulbs for cold acclimation that depended on hybrid x thermal treatment. Plant leaf area and flower stem characteristics were similar in the hybrids and were unaffected by the bulb-preparation procedure.