Abstract
Regarding the fact that cell shape indicates cell health and is of particular importance in the evaluation of new therapies, in this study, stem cell deformation during Atmospheric Pressure Plasma (APP) treatment was investigated. Given that, cell deformation is a warning of cell damage, it is therefore expected that APP-based therapy, a new modern technology that is expanding worldwide, will not lead to the deformation of normal cells. Here, the stem cells exposed to Helium-fed jet plasma, with two di erent powers of 15 and 25W. Moreover, the duration of exposure was changed (30, 50, 70, and 90 seconds) to determine the most appropriate exposure time and voltage, which maintains stem cells’ health condition. First of all, it was found that cold plasma at low power does not change the shape and elongation of stem cells. Besides, it was found that if the power of a cold plasma source is 25W, it will raise cell growth rate. In this paper, the gas ow rate of the helium plasma jet was set to 3.9 liters per minute, and a plasma source frequency of 30kHz was selected.
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