Abstract
Serial serum samples from 35 renal allograft recipients were screened for cold and warm cytotoxic antibodies using a two-stage microlymphocytotoxicity test with three incubation temperatures. Both cold and warm antibodies were reactive at 22°C. The critical temperatures which distinguished the two classes of antibodies were 15°C and 37°C. Cold antibodies were detected in sera from 15 patients, 10 patients had warm antibodies, and the remaining 10 had a mixture of cold and warm antibodies. All the patients were cytotoxin-positive after transplantation, although not all had preformed lymphocytotoxins. At one year 14 of the patients with cold autoantibodies had functioning grafts, but only 3 of the 10 patients with warm antibodies had kidneys functioning. This difference was statistically highly significant. There was no difference in graft survival among patients with warm antibodies and those with a mixture of cold and warm antibodies.
Published Version
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