Cold acclimation increases Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae) survival during exposure to freezing temperatures.

Abstract

The Asian citrus psyllid, Diaphorina citri, is the vector of the pathogenic bacteria that causes Huanglongbing, the most devasting disease affecting citrus worldwide. As cultivation of citrus tends to expand northward, the tolerance of psyllids to freezing temperatures needs to be investigated. While mortality of D. citri to cold temperature has been previously studied, much less is known regarding the acclimation potential of psyllids to cold temperatures. We first evaluated cold resistance of D. citri depending on color morph and sex. Subsequently, we compared mortality of D. citri at -4°C for 10 hours between unacclimated psyllids, psyllids that were gradually acclimated to cold over 1 or 2 weeks, and psyllids intermittently acclimated also for 1 or 2 weeks. We did not find major differences in cold resistance between color morphs and sex. We found that after 1 week of gradual acclimation, the survival of psyllids at -4°C increased to 86%, compared to 20% in the control group. Survival did not increase after the second week of gradual acclimation. Although intermittent acclimation improved survival compared to the control group, it was less effective than gradual acclimation with a survival at 30% and 70% after 1 and 2 weeks of acclimation, respectively, although this difference with gradual acclimation was not significant at week 2. These data show that gradual cold acclimation allows D. citri to survive brief periods below freezing. It may serve as a mechanism responsible for increasing northern establishment of this pest.