Abstract
The expression and activity of phospholipase A 2 (PLA 2) isoforms were investigated in primary cultures of striatal astrocytes. The calcium ionophore A23187 together with the protein kinase C activator phorbol ester was the most potent stimulus in eliciting [ 3H]arachidonic acid release in the extracellular medium. Reverse transcription coupled to polymerase chain reaction (RT-PCR) showed the presence of the 85 kDa cytosolic PLA 2 mRNA and the 14 kDa secretory PLA 2 mRNA in untreated astrocytes. Immunoblot experiments with isoform-specific antibodies showed the presence of the cytosolic PLA 2 in untreated astrocytes, while the secretory PLA 2 was detected only in lipopolysaccharide-treated astrocytes. These data suggest that the two PLA 2 isoforms expressed in striatal astrocytes might play different roles in cellular processes mediated by astrocytes.
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