Abstract
To determine if lysophosphatidylcholine (lysoPC) is able to induce proinflammatory changes in monocytes, its ability to stimulate arachidonic acid (AA) release, a product of phospholipase A2 (PLA 2) activity, has been analyzed. LysoPC increased AA release in THP-1 and Mono Mac6 cells in a time- and concentration-dependent manner. The monocytes expressed both secretory and cytosolic PLA 2 enzymes and AA release was strongly reduced by cellular pretreatment with different PLA 2 inhibitors and by pertussis toxin, an inhibitor of G i-protein activation. This indicates that both cytosolic and secretory PLA 2 enzymes regulate specific lysoPC receptor-induced AA release, suggesting lysoPC participation in monocyte proinflammatory activation.
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