Abstract
A human norovirus (HuNoV) strain was obtained from a patient with acute gastroenteritis, and its complete coding sequence was determined. The coding-complete viral genome, with three open reading frames, was 7,565 bp long, with a GC content of 49.9%. The genotype of the HuNoV strain obtained in this study was identified as GII.p12_GII.3.
Highlights
Norovirus, a viral pathogen that causes gastroenteritis in humans, results in 19 to 21 million total illnesses and 56,000 to 71,000 hospitalizations every year in the United States (1)
When the coding-complete sequence of the CAU140599 strain was compared to the sequences deposited in GenBank by phylogenetic analysis using Geneious version 10.2.6, it presented 94.40% nucleotide identity to strain GII/Hu/JP/ 2000/GII.P12_GII.12/Saitama/KU16
open reading frame 2 (ORF2) of the CAU140599 strain was classified in the GII.[3] genotype based on phylogenetic analysis (Fig. 1C)
Summary
A viral pathogen that causes gastroenteritis in humans, results in 19 to 21 million total illnesses and 56,000 to 71,000 hospitalizations every year in the United States (1). (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and DIAMOND version 0.9.21 (https://github .com/bbuchfink/diamond), with a cutoff E value of 1.0EϪ5. The coding-complete genome of NoV, containing three ORFs, had 19,641.2ϫ coverage with 1,001,204 reads.
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