Coding sequences of the MHC ii β chain of homozygous rainbow trout (Oncorhynchus mykiss) 1

Abstract

Six lines of homozygous rainbow trout ( Oncorhynchus mykiss) from different genetic and geographical backgrounds have been produced as aquatic models for biomedical research by the chromosome set manipulation techniques of androgenesis and gynogenesis Messenger RNA from spleens was extracted and the MHC II B cDNA sequences amplified by RT–PCR were cloned into plasmids Sequences of the MHC II β2 domains were highly conserved between the different plasmids from the same and different lines of trout Most of the variability among sequences was found in the amino terminal half of the β1 domain which corresponds with the peptide binding region of the MHC II molecule This diversity suggests that the different lines of trout may exhibit differences in immune response Rainbow trout MHC II B sequences were similar to the MHC II B sequences of the Pacific salmon ( O gorbuscha O tshawytscha O nerka O masou O kisutch) Southern blot analysis performed on the restricted DNA of the OSU and Hot Creek trout and the doubled haploid progeny produced by androgenesis from OSU×Hot Creek hybrids indicates that two distinct genes encode the MHC II B sequences and that these genes are unlinked © 1999 Elsevier Science Ltd All rights reserved