Abstract
Multidrug combination therapy for pancreatic cancer is widely applied in clinical practice. In this study, we used phenylboronic acid and polyethylene glycol as materials of nanomicelles, loaded with the PI3K/mTORC1 dual inhibitor PF04691502 (PF) to inhibit the resistance and metastasis of pancreatic cancer and increase the sensitivity of doxorubicin (DOX). We prepared the PPD nanoparticles (NPs) with a small PDI and a uniform morphology by controlling the DOX substitution degree (size of 164.8 ± 3.6 nm and zeta potential of −16.9 ± 0.4 mV). We determined the rates of PF and materials through the combination experiment of free drugs and the obtained PF@PPD NPs (size of 200.8 ± 2.6 nm and zeta potential of −13.9 ± 0.3 mV). The drug loadings of DOX and PF in the nanomicelle were 14.8 ± 0.4% and 9.5 ± 0.3%, respectively. And the drug release in vitro was slow (29.17 ± 2.00% for DOX and 39.22 ± 2.49% for PF). The cell assay showed that the NPs had a good curative effect and migration on BxPC‐3 cells, and it could be continuously taken up by cells. The PF@PPD NPs displayed a dose‐dependent cytotoxicity with less cell viability (20.38 ± 1.11%) and higher uptake in BxPC‐3 cells compared with the free drug. The combined medication or PF@PPD NPs reduced tumor metastasis, indicating that PF@PPD NPs had the potential for clinical application.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.