Abstract
In this study, in order to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and glucose isomerase (GI) were immobilized together with cross-linked enzyme aggregates (CLEAs), and named as 'combi-CLEAs'. The values of Vmaxand Kmfrom the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12 U g-1 and 0.1157 mg ml-1 in optimum conditions, respectively. After 50 uses, it was observed that the remaining activity of combi-CLEAs [GA + PUL + GI] was preserved at 46%. The thermal stability of combi-CLEAs [GA + PUL) + GI] was observed to be 44% and 30%, respectively, at 60 and 80 ° C after 24 hours. At the end of 30 days, the storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and 13% at the temperatures of 4 and 25°C, respectively.
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