Abstract
In this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (MNPs) using metal coordinated hydrogel nanofibers. Candida rugosa lipase (CRL) was selected as guest protein. For good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (CA-Fe3O4 NPs) have been widely used for immobilizing enzymes. As a result, the relative activity of CA-Fe3O4@Zn/AMP nanofiber-immobilized CRL increased by 8-fold at pH 10.0 and nearly 1-fold in a 50 °C water bath after 30 min, compared to free CRL. Moreover, the immobilized CRL had excellent long-term storage stability (nearly 80% releative activity after storage for 13 days). This work indicated that metal-nucleotide nanofibers could efficiently co-immobilize enzymes and MNPs simultaneously, and improve the stability of biocatalysts.
Highlights
Enzymes have been considered as potential substitutes for traditional chemical catalysts due to their environmental friendliness [1], low cost [2], high specificity [3], and mild reaction conditions [4]
Various materials have been used for immobilizing enzymes [13], such as carboxyl-functionalized graphene oxide [14], polyurethane foam support [15], SBA-15 [16], and chitosan [17]
The morphology of CA-Fe3O4 NPs in Zn/Adenosine -monophosphate (AMP) nanofibers was analyzed by transmission electron microscope (TEM)
Summary
Enzymes have been considered as potential substitutes for traditional chemical catalysts due to their environmental friendliness [1], low cost [2], high specificity [3], and mild reaction conditions [4]. The difficult most researchers select porous nanomaterials as immobilized supports nowadays. Recovery of ofnanostructured nanostructuredimmobilized immobilized enzymes by using magnets the separation medium. Magnetic nanoparticles, such as iron oxide and ferric oxide, have been successfully introduced [24,25]. NMCPs have excellent self-assemble and can entrap various guest molecules, 2+ /AMP CPs could efficiently immobilize proteins and nanoparticles. We recently demonstrated that Zn /AMP CPs could efficiently single enzymes or enzymes multi-enzymes with high stability [43]. Because of their nanofibrous properties, immobilize single or multi-enzymes with high stability Because of their nanofibrous it’s very difficult to recover immobilized enzymes byenzymes traditional methods. CA-Fe33O44@Zn/AMP NPs have high stability activity and good recovery.
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