Abstract
This study discusses the molecular mechanism of co-culture of MSCs with miR-496 in the EMT of OSCC. miR-496 expression in OSCC tissues and cells was detected by qRT-PCR. The MSCs+si-NC, MSCs+pc-DNA-NC, MSCs+si-miR-496, MSCs+pc-DNA-miR-496 was transfected into SCC-PKUs to analyze cell apoptosis, proliferation and clone formation as well as the expression of proteins related with EMT. miR-496 in OSCC tissues was elevated and positively related with the clinical stage of OSCC patients. The cell activity and invasive ability of SCC-PKU was increased after co-culture of MSCs and pc-DNA-miR-496 along with increased expression of EMT-related proteins. However, all above changes were reversed after treatment with MSCs and si-miR-496. In conclusion, co-culture of MSCs and miR-495 prompts the occurrence and development of OSCC through regulating EMT processes, indicating that they might be novel targets for the treatment of OSCC.
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