Co-application of TiO2 nanoparticles and hyperaccumulator Brassica juncea L. for effective Cd removal from soil: Assessing the feasibility of using nano-phytoremediation

Abstract

Nano-phytoremediation is anticipated as a potential technology for the remediation of heavy metals from soil sites. This study evaluated the feasibility of using titanium dioxide nanoparticles (TiO2 NPs) at various concentrations (0, 100, 250, 500 mg/kg) along with a hyperaccumulator, Brassica juncea L., for effective removal of Cadmium (Cd) from the soil. Plants were grown for a whole life cycle in soil containing 10 mg/kg of Cd and spiked TiO2 NPs. We analyzed the plants for Cd tolerance, phytotoxicity, Cd removal, and translocation. Brassica plants displayed high Cd tolerance with a significant increase in plant growth, biomass, and photosynthetic activity in a concentration-dependent manner. Cd removal from the soil at TiO2 NPs concentrations of 0, 100, 250, and 500 mg/kg treatment was 32.46%, 11.62%, 17.55%, and 55.11%, respectively. The translocation factor for Cd was found to be 1.35, 0.96, 3.73, and 1.27 for 0, 100, 250, and 500 mg/kg concentrations. The results of this study indicate that TiO2 NPs applications in the soil can minimize Cd stress in plants and lead to its efficient removal from soil. Thus, the association of nanoparticles with the phytoremediation process can lead to good application prospects for the remediation of contaminated soil.