Abstract
A human hepatoma (HepG2) cell line library was screened with an oligonucleotide probe for macrophage stimulating protein (MSP) to clone an MSP cDNA. Deduced sequences of isolated clones were compared with peptide fragment sequences of MSP. MSP9 cDNA encoded most of the known sequence of MSP except for a small segment of the 5' end of the open reading frame. Consequently, a hybrid 2300-base pair cDNA that encoded the complete MSP amino acid sequence was constructed from 2 clones. Culture fluid from COS-7 cells transfected with this full-length MSP cDNA had MSP biological activity, and the expressed MSP was detected by immunoprecipitation with antibody against native MSP. The deduced amino acid sequence of MSP includes 4 kringle domains, which have been found in hepatocyte growth factor and several proteins of the blood coagulation system. Among them, MSP has the highest sequence similarity to hepatocyte growth factor (45% identity). The MSP cDNA hybridized strongly to mRNA from liver, and to a lesser extent to mRNA from kidney and pancreas, suggesting that a cell type in the liver is the source of MSP. Several cloned and sequenced MSP cDNAs had insertions or deletions, suggesting that alternatively spliced MSP mRNAs may occur. This was reflected in Northern blots probed with an MSP cDNA, which showed more than one mRNA species. Furthermore, although the gene coding for MSP is on chromosome 3, the sequence of one of the cDNAs was identical with a unique sequence in chromosome 1, indicating that there may be a family of MSP genes, located on chromosomes 3 and 1.
Highlights
From the $ImmunopathologySection, Laboratory of Immunobwlogy, and the BGenetics Section, Laboratory of Viral Carcinogenesis, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702
A human hepatoma (HepG2) cell line library was nally given to a human serum protein that makes resident screened withan oligonucleotideprobe formacrophage peritoneal mouse macrophages capable of responding to the stimulatingprotein (MSP) to clone an Macrophage stimulating protein (MSP) cDNA. chemoattractant C5a (1, 2)
Culture fluid froCmOS7 cells transfected with this full-lengMthSP cDNA had MSP biological activity, and the expressed MSP was detected by immunoprecipitation wiathntibody against nativeMSP
Summary
A hybrid 2300-base pair cDNA that encoded the complete MSP amino acid sequence was constructed fro2mclones. Several cloned and sequenced MSP cDNAs had insertions or deletions, suggesting that alternatively spliced MSP mRNAsmay occur. This was reflected in Northern blots probed with anMSP cDNA, which showed more than one mRNA species. Because of alternative splicing that resulted in insertions or deletions, it was necessary to isolate and sequence several cDNAs to deduce the complete open reading frame for MSP. Immunoprecipitation of the COS-7 cell product revealed protein bands of the expected a family of MSP genes, located on chromosomes 3 and mass, and thesecreted MSP was biologically active. Sequence andrestrictionsite analyses show thattheMSP gene is located on chromosome 3
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