Abstract
ABSTRACTYacon is a perennial plant grown in the Andes, which was introduced into Japan for its high accumulation of inulin-type fructan in tuberous roots in 1980s. We isolated a 1-SST gene (Yc 1-SST) from cDNA library derived from yacon mature tuberous root. The coding region of the gene is 1920bp long, and its deduced protein consists of640 amino acids with a predicted molecular mass of 72kDa. The deduced amino acid sequence of Yc 1-SST shares 87% identity with 1-SST (EC 2.4.1.99) from Jerusalem artichoke (Helianthus tuberosus L.). A functional analysis for Yc 1-SST was performed by transforming into rice (Oryza sativa L. cv.Nipponbare) and compared with rice transformed with 1-SST gene of Jerusalem artichoke (Ja 1-SST) under the regulation of the cauliflower mosaic virus (CaMV) 35S promoter. Transgenic rice overexpressing Yc 1-SST produced fructan such as 1-kestose (GF2), 1-nystose (GF3) and 1-fructosylnystose (GF4), which showed the same activity as Ja 1-SST.
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