Abstract
An extremely high-efficiency technique is described for cloning double-stranded (ds) cDNAs in Escherichia coli. The method which uses two synthetic oligonucleotide linkers rather than one results in approx. 200–500 recombinant clones per ng of ds cDNA. This technique was used to clone a cDNA comprising 95% of the full length of the mRNA for α 2u globulin a male rat liver protein which represents approx. 1% of hepatic messenger RNA. The cloned probe was applied to study the complex hormone controls of α 2u globulin mRNA in male and female rats.
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