Abstract
Cloning forever
Highlights
As an example, a company had difficulty expressing an enzyme critical to the development of a new line of services
Just a matter of adding solubility tags and possibly a chaperone to help with the folding
Why didn't they get the right plasmid the first time? Why is it that they didn't put a solubility tag and a chaperone to begin with? What makes them think that the plasmid is going to work when the previous one did not? If the plasmid does not work, how many more iterations will be needed? How long will it take and how much money will it cost to deliver what the company needs?
Summary
CITATION: Jean Peccoud, Cloning forever, The Winnower 2:e143018.86995 , 2015 , DOI: 10.15200/winn.143018.86995 © Peccoud This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and redistribution in any medium, provided that the original author and source are credited.
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