Cloning and sequence of the human obesity -related neuropeptide Y receptor Y5 gene

Abstract

Objective To clone and identify human neuropeptide Y receptor Y5 (NPYSR), one of the human obesity - related genes.Methods The cDNA of NPYSR gene was amplified from RNA of the human fat by reverse transcription polymerase chain reaction (RT -PCR) . The PCR products were recombined with pET28a + vector, positive clones were selected and DNA sequence analysis was conducted for the identification, followed by sequence analysis and homology comparison with that reported in Genebank after sequencing. Results A DNA fragment of about 1300bp was obtained by RT- PCR, the cDNA sequence of the recombinant plasmid of pET28a + with the fragment was analyzed, and the result showed that the cloned DNA fragment was just human NPYSR cDNA. The cloned gene encoded 445 amino acids with a molecular weight of 50KD. The nuclectide sequence homology of the cloned NPY5R was 100% in comparison with thai reported in GeneBank. Condusions The sequence of NPYSR cDNA that we have successfully cloned is the same as that of NPYSR in C, eneBank, which lays the foundation for further studies of gene expression of NPYSR and the interrelationship of NPYSR and the genesis, development and transformation d obesity by means of molecular biology. Key words: Obesity; Neuropeptide Y receptor Y5 ; Gene cloning; DNA sequence analysis