Abstract
Carotenoids are an important group of precursors of volatile flavour compounds in tea and phytoene synthase (PSY) is a key enzyme during the biosynthesis pathway of carotenoids. A cDNA encoding PSY in tea shoot [Camellia sinensis (L.) O. Kuntze] was cloned and sequenced in the present study. The obtained tea PSY cDNA was 1296 bp with an open reading frame of 987 bp. The predicted protein displayed a sequence of 329 amino acids. The phylogenetic tree showed that the tea PSY was clustered closely to the PSY of Adonis palaestina, with an overall amino acid identity of 90%.
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