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Abstract
A thi2( pho6) mutant of Saccharomyces cerevisiae, defective in the expression of structural genes for thiamin-repressible acid phosphatase and enzymes involved in thiamin biosynthesis, was found to retain sufficient thiamin transport activity. The transport activity was repressed by thiamin in growth medium. We isolated from a S. cerevisiae genomic library two hybrid plasmids, pTSR 1 and pTSR2, containing 10.2- and 12.0-kilobase (kb) DNA fragments, respectively, which complement the thi2( pho6) mutation of S. cerevisiae. This gene was localized on a 6.0-kb ClaI- ClaI fragment in the subclone pTSR3. Complementation of the enzyme activities for thiamin metabolism in the thi2( pho6) mutant transformed by some plasmids with the THI2( PHO6) gene was also examined.
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