Abstract
A cloned cDNA was generated to a transcript for a major ovarian polypeptide (200 kDa) of the South American white shrimp, Penaeus vannamei. The cloned cDNA hybridized to a single transcript in ovaries but not to RNA from the hepatopancreas or muscle. For immunodetection and quantitation, a monospecific polyclonal antibody was raised against the cDNA translation product expressed in bacteria. The antibody was used to show that the 200 kDa ovarian polypeptide accumulated in cortical granules during ovarian development to comprise ∼11% of the total ovarian protein and disappeared during early embryonic development. These studies begin to explain a gene-product relationship essential for reproduction in P. vannamei.
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