Clonal Structure, Virulence Factor-encoding Genes and Antibiotic Resistance of Escherichia coli, Causing Urinary Tract Infections and Other Extraintestinal Infections in Humans in Spain and France during 2016.

Saskia-Camille Flament-Simon,María Pilar Alonso,Marion Duprilot,Miguel Blanco,Isidro García-Meniño,Jorge Blanco,Jesús E Blanco,Noémie Mayer,Marie-Hélène Nicolas-Chanoine,Vanesa García

doi:10.3390/antibiotics9040161

Abstract

Escherichia coli is the main pathogen responsible for extraintestinal infections. A total of 196 clinical E. coli consecutively isolated during 2016 in Spain (100 from Lucus Augusti hospital in Lugo) and France (96 from Beaujon hospital in Clichy) were characterized. Phylogroups, clonotypes, sequence types (STs), O:H serotypes, virulence factor (VF)-encoding genes and antibiotic resistance were determined. Approximately 10% of the infections were caused by ST131 isolates in both hospitals and approximately 60% of these infections were caused by isolates belonging to only 10 STs (ST10, ST12, ST58, ST69, ST73, ST88, ST95, ST127, ST131, ST141). ST88 isolates were frequent, especially in Spain, while ST141 isolates significantly predominated in France. The 23 ST131 isolates displayed four clonotypes: CH40-30, CH40-41, CH40-22 and CH40-298. Only 13 (6.6%) isolates were carriers of extended-spectrum beta-lactamase (ESBL) enzymes. However, 37.2% of the isolates were multidrug-resistant (MDR). Approximately 40% of the MDR isolates belonged to only four of the dominant clones (B2-CH40-30-ST131, B2-CH40-41-ST131, C-CH4-39-ST88 and D-CH35-27-ST69). Among the remaining MDR isolates, two isolates belonged to B2-CH14-64-ST1193, i.e., the new global emergent MDR clone. Moreover, a hybrid extraintestinal pathogenic E.coli (ExPEC)/enteroaggregative isolate belonging to the A-CH11-54-ST10 clone was identified.

Highlights

Escherichia coli is the leading cause of urinary tract (UTI) and bloodstream infections
None of the genes specific for the different diarrheagenic E. coli pathovars was detected in the remaining 195 isolates. This is the first study conducted in Spain in which the clonal structure of extraintestinal pathogenic E. coli was analyzed from clinical E. coli non-redundant and consecutively isolated
We concluded that approximately 10% of the extraintestinal E. coli infections that had occurred in 2016 in the two studied hospitals were caused by ST131 isolates, and approximately 60% of these infections were caused by isolates belonging to only 10 sequence types (STs) (ST10, ST12, ST58, ST69, ST73, ST88, ST95, ST127, ST131, ST141)

Summary

Introduction

Escherichia coli is the leading cause of urinary tract (UTI) and bloodstream infections. Most infections like this are due to isolates of pathotypes known as extraintestinal pathogenic E. coli (ExPEC). Numerous virulence genes have been associated with isolates causing extraintestinal infections, such as adhesins, toxins, siderophores and capsular antigens, that. Antibiotics 2020, 9, 161 antigens, that enable them to colonize host surfaces, capture available iron, injure host tissues and avoid defense systems. Enablehost them to colonize host surfaces, capture available iron, injure host tissues and avoid host Thesystems. Treatment of these infections has been seriously complicated by the appearance of defense multidrug-resistant isolates and especially the rapid complicated disseminationby of extended-spectrum. Of these infections has beenbyseriously the appearance of β-lactamase-producing

Methods

Discussion

Conclusion