Clonal analysis of bone marrow stromal cells in multiple exostotic chondrodysplasia and systemic osteoporosis: features of cell cloning efficiency

Abstract

We studied the clonal features of bone marrow stromal cells obtained by biopsy of the iliac wing of patients with systemic skeletal diseases: multiple exostotic chondrodysplasia (16 patients) and systemic osteoporosis (24 patients). The proliferative potential of cells was investigated, the main indicator of which is the efficiency of cloning. We analyzed the induction-morphogenetic relationship between the efficiency of cloning and biological inducers contained in the autologous and added suspension of bone marrow cells (the so-called feeder). The rapid growth and proliferation of exostoses in multiple exostotic chondrodysplasia is apparently due to both the increased proliferative activity of the cultured cells themselves, and (especially) the increase in the growth-stimulating effect of the autologous "feeder", which distinguishes fast-growing exostoses from the so-called calm ones, in which the proliferative activity itself cells are increased (but to a lesser extent), and no enhancement of the effect of the autofeed is detected. In systemic osteoporosis, a sharp decrease in the efficiency of cloning of the target cells themselves has been established, which is probably associated with a weakening of their colony-forming properties and / or a decrease in their number in the explanted suspension.